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Campylobacter Identification - Isolate

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Aliases

Campy

Test Name
Campylobacter Identification - Isolate
Test Code

CAMPYID

Methodology

MALDI-TOF (matrix-assisted laser desorption ionization-time of flight) mass spectrometry

Overview/Supplementary

This test identifies Campylobacter species.

Turn Around Time

3-5 days

Specimen Requirements

Isolates: Actively growing culture on media that support the growth of the bacterial isolate submitted.

Special Considerations

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html)

Transport/Stability

Store at ambient (8°C to 25°C) temperature. Ship at ambient (8°C to 25°C) or refrigeration (2°C to 8°C) temperature.

Interface/Codes

LOINC: 20738-1

Test Resource ID
876

Campylobacter Culture - Stool

Enviado por StewaD1 el
Aliases

Campy

Test Name
Campylobacter Culture - Stool
Test Code

CAMPYCU

Methodology

MALDI-TOF (matrix-assisted laser desorption ionization-time of flight) mass spectrometry

Overview/Supplementary

This test isolates Campylobacter for molecular characterization testing.

Turn Around Time

3-7 days

Specimen Requirements

Specimens: Stool or rectal swabs preserved in Para-Pak C&S, modified Cary-Blair or equivalent transport media. 

Minimum volumes: 
Rectal swab: shows visible trace of fecal material and is immersed in transport media.
Stool specimen: transport media vial filled with approximately 5 ml of stool. 

Special Considerations

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html)

Transport/Stability

Store at ambient (8°C to 25°C) temperature. Ship at ambient (8°C to 25°C) or refrigeration (2°C to 8°C) temperature.

Interface/Codes
Test Resource ID
875

Burkholderia mallei/pseudomallei PCR - Specimen

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Aliases

Glanders, Melioidosis, Whitmore's disease

Test Name
Burkholderia mallei/pseudomallei PCR - Specimen
Test Code

BURKPCRSPEC

Methodology

Real-time PCR

Overview/Supplementary

This test detects Burkholderia mallei/pseudomallei from clinical specimens. MDH will determine if additional tests are required, including culture. Francisella tularensis real-time PCR and Brucella spp. real-time PCR assays may also be performed on isolates submitted for Burkholderia mallei/pseudomallei testing.

Turn Around Time

1-3 days

Specimen Requirements

Specimens: Call IDL emergency response on-call phone 612-282-3723 for requirements for specimen submission or additional requirements.

Special Considerations

Call IDL emergency response on-call phone 612-282-3723 before ordering this test.

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html)

Transport/Stability

Ship whole blood or serum at refrigeration (2°C to 8°C) temperature.

Interface/Codes

LOINC: 41628-9

Test Resource ID
873

Burkholderia mallei/pseudomallei PCR - Isolate

Enviado por StewaD1 el
Aliases
Glanders, Melioidosis, Whitmore's disease
Test Name
Burkholderia mallei/pseudomallei PCR - Isolate
Test Code
BURKPCRISO
Methodology
Real-time PCR
Overview/Supplementary
This test identifies Burkholderia mallei/pseudomallei from isolates. MDH will determine if additional tests are required. Francisella tularensis real-time PCR and Brucella spp. real-time PCR assays may also be performed on isolates submitted for Burkholderia mallei/pseudomallei testing.
Turn Around Time
1-3 days
Specimen Requirements
Isolates: Actively growing culture on media that support the growth of the bacterial isolate submitted.
Special Considerations

Call IDL emergency response on-call phone 612-282-3723 before ordering this test.

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html)

Transport/Stability

Store and ship at ambient (8°C to 25°C) or refrigeration (2°C to 8°C) temperature.

Interface/Codes
LOINC: 41628-9
Test Resource ID
874

Brucella PCR - Specimen

Enviado por StewaD1 el
Aliases

Brucellosis

Test Name
Brucella PCR - Specimen
Test Code

BRUPCRSPEC

Methodology

Real-time PCR

Overview/Supplementary

This test detects Brucella spp. from clinical specimens. MDH will determine if additional tests are required, including culture. Francisella tularensis real-time PCR and Burkholderia mallei/pseudomallei real-time PCR assays may also be performed on isolates submitted for Brucella spp. testing.

Turn Around Time

1-3 days

Specimen Requirements

Specimens: Call IDL emergency response on-call phone 612-282-3723 for requirements for specimen submission.

Special Considerations

Call IDL emergency response on-call phone 612-282-3723 before ordering this test.

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html)

Transport/Stability

Store and ship at ambient (8°C to 25°C) or refrigeration (2°C to 8°C) temperature.

Interface/Codes

LOINC: 41626-3

Test Resource ID
871

Brucella PCR - Isolate

Enviado por StewaD1 el
Aliases

Brucellosis

Test Name
Brucella PCR - Isolate
Test Code

BRUPCRISO

Methodology

Real-time PCR

Overview/Supplementary

This test identifies Brucella spp. from isolates. MDH will determine if additional tests are required. Francisella tularensis real-time PCR and Burkholderia mallei/pseudomallei real-time PCR assays may also be performed on isolates submitted for Brucella spp. testing.

Turn Around Time

1-3 days

Specimen Requirements

Isolates: Actively growing culture on media that support the growth of the bacterial isolate submitted.

Special Considerations

Call IDL emergency response on-call phone 612-282-3723 before ordering this test. 

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html)

Transport/Stability

Store and ship at ambient (8°C to 25°C) or refrigeration (2°C to 8°C) temperature.

Interface/Codes

LOINC: 41626-3

Test Resource ID
872

Bordetella PCR

Enviado por StewaD1 el
Aliases
Bordetella parapertussis, Bordetella pertussis, Pertussis, Whooping cough
Test Name
Bordetella PCR
Test Code
BORDPCR
Methodology
Real-time PCR
Overview/Supplementary
This test detects B. pertussis, B. parapertussis, and B. holmesii. Bordetella pertussis toxin (ptxS1) PCR results are reported when applicable.
Turn Around Time
1-3 days
Specimen Requirements

Isolates: Cultures on Regan Lowe transport medium.

Specimens: Nasopharyngeal swab (dry or in VTM, such as M4, UTM, Alethia Pertussis transport buffer) or upper respiratory specimens

Preferred volume: 2 mL
Minimum volume: 200 µL

Special Considerations
States submitting to the VPD Reference Center should contact Health.VPD.submissions@state.mn.us for additional information.
Transport/Stability

Store and ship at refrigeration (2°C to 8°C) temperature within 4 days of collection. Alternatively, ship at ambient (8°C to 25°C) temperature within 7 days of collection.

Interface/Codes
LOINC: 62426-2
Test Resource ID
869

Blood Parasite Smear Giemsa Stain

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Aliases

Babesia, Malaria, Microfilaria, Plasmodium, Trypanosome

Test Name
Blood Parasite Smear Giemsa Stain
Test Code

BLDSMEAR

Methodology

Microscopic exam

Overview/Supplementary

This test detects blood parasites (e.g., Babesia, microfilariae, Plasmodium, trypanosomes). Parasitemia will be reported, as appropriate. Plasmodium PCR - Blood will be performed if Plasmodium sp. is suspected.

Turn Around Time

1-3 days

Specimen Requirements

Specimens: Thick and thin blood film smears from whole blood, EDTA whole blood 

Preferred volume: 1 mL
Minimum volume: 200 µL

Special Considerations

Do not allow EDTA tube to sit for prolonged time with cap off at room temperature.

Parasite and red blood cell morphology can become distorted.

Transport/Stability

Store and ship at ambient (8°C to 25°C) or refrigerated (2°C to 8°C) temperature.

Interface/Codes

LOINC: 51714-4

Test Resource ID
868

Blastomyces & Histoplasma PCR - Isolate

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Aliases

Blastomyces dermatitidis, Blastomyces gilchristii, Fungal PCR, Histoplasma capsulatum

Test Name
Blastomyces & Histoplasma PCR - Isolate
Test Code

BLASTOHISTOPCR

Methodology

Real-time PCR

Overview/Supplementary

This test detects Blastomyces dermatitidis, Blastomyces gilchristii, and Histoplasma capsulatum. MDH will determine if additional tests are required, including fungal identification.

Turn Around Time

1-3 days

Specimen Requirements

Isolates: Actively growing culture on media that support the growth of the fungal isolate submitted.

Use slant with screw cap or ensure plates are sealed.

Special Considerations

None

Transport/Stability

Store and ship at ambient (8°C to 25°C) or refrigerated (2°C to 8°C) temperature.

Interface/Codes

LOINC: 15378-3

Test Resource ID
867

BioFire NGDS Warrior Panel

Enviado por StewaD1 el
Aliases

Bacillus anthracis, Ebola virus, Marburg virus, Coxiella burnetii, Yersinia pestis, Francisella tularensis

Test Name
BioFire NGDS Warrior Panel
Test Code

WARRIOR

Methodology

Real-time multiplex PCR by BioFire FilmArray

Overview/Supplementary

This test identifies Bacillus anthracis, Coxiella burnetii, Ebola virus, Francisella tularensis, Marburg virus, and Yersinia pestis using a FilmArray PCR. Results from this assay are presumptive only.

Additional testing may be performed at CDC.

Turn Around Time

1-3 days

Specimen Requirements

Specimens: Two EDTA blood tubes, 5 mL Vacutainer (or equivalent).

The second tube is required to send to CDC for additional testing, if needed.

Special Considerations

Contact Infectious Disease Epidemiology Prevention and Control (IDEPC) at 651-201-5414 or 877-676-5414 before requesting this test.

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html)

Transport/Stability

Call IDL emergency response on-call phone 612-282-3723 for shipping conditions and additional specimen submission requirements.

Interface/Codes
Test Resource ID
866