Pasar al contenido principal

ETEC (Enterotoxigenic Escherichia coli) PCR Panel

Enviado por StewaD1 el
Aliases
Test Name
ETEC (Enterotoxigenic Escherichia coli) PCR Panel
Test Code

ETEC

Methodology

Conventional PCR, Real-time PCR

Overview/Supplementary

This test detects enterotoxigenic Escherichia coli (ETEC) from clinical specimens using conventional culture and real-time PCR methods.

Turn Around Time

3-5 days

Specimen Requirements

Specimens: Stool or rectal swabs preserved in Para-Pak C&S, modified Cary-Blair or equivalent transport media. 

Minimum volumes: 
Rectal swab: shows visible trace of fecal material and is immersed in transport media.
Stool specimen: transport media vial filled with approximately 5 ml of stool. 

Special Considerations

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html).

Transport/Stability

Store at ambient (8°C to 25°C) temperature. Ship at ambient (8°C to 25°C) or refrigeration (2°C to 8°C) temperature.

Interface/Codes
Test Resource ID
897

Escherichia coli O157 Identification - Isolate

Enviado por StewaD1 el
Aliases
STEC, VTEC
Test Name
Escherichia coli O157 Identification - Isolate
Test Code
O157ID
Methodology
Biochemicals, Culture, Latex agglutination, MALDI-TOF (matrix-assisted laser desorption ionization-time of flight) mass spectrometry
Overview/Supplementary
This test detects the presence of Escherichia coli O157:H7 from bacterial isolates. E. coli O157:H7 is a known cause of moderate-severe diarrhea and bloody diarrhea with ~3-20% of cases going on to develop hemolytic uremic syndrome (HUS).
Turn Around Time
3-5 days
Specimen Requirements
Isolates: Actively growing culture on media that support the growth of the bacterial isolate submitted.
Special Considerations
This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html).
Transport/Stability

Store at ambient (8°C to 25°C) temperature. Ship at ambient (8°C to 25°C) or refrigeration (2°C to 8°C) temperature.

Interface/Codes
LOINC: 44090-9
Test Resource ID
896

EPEC (Enteropathogenic Escherichia coli) PCR Panel

Enviado por StewaD1 el
Aliases

EPEC, E. albertii, Escherichia albertii

Test Name
EPEC (Enteropathogenic Escherichia coli) PCR Panel
Test Code

RDRSPEC

Methodology

Real-time PCR

Overview/Supplementary

EPEC are a group of E. coli defined by a common virulence factor, intimin (eae), which is carried by other pathogenic E. coli like STEC (Shiga toxin-producing E. coli) and Escherichia albertii.

This test detects Escherichia albertii from clinical specimens.

Turn Around Time

Not Applicable

Specimen Requirements

Specimens: Stool or rectal swabs preserved in Para-Pak C&S, modified Cary-Blair or equivalent transport media. 

Minimum volumes: 
Rectal swab: shows visible trace of fecal material and is immersed in transport media.
Stool specimen: transport media vial filled with approximately 5 ml of stool. 

Special Considerations

For surveillance use only. Results not reported back to submitter.

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html).

Transport/Stability

Store at ambient (8°C to 25°C) temperature. Ship at ambient (8°C to 25°C) or refrigeration (2°C to 8°C) temperature.

Interface/Codes
Test Resource ID
895

Enterovirus Genotype by VP1 NGS

Enviado por StewaD1 el
Aliases

Enterovirus typing, EV68

Test Name
Enterovirus Genotype by VP1 NGS
Test Code

EVVP1-NGS

Methodology

Next generation sequencing

Overview/Supplementary

This test characterizes enteroviruses, including coxsackieviruses, echoviruses, polioviruses, and rhinoviruses by VP1 gene sequencing on high viral load enterovirus positives tested with the Rhinovirus and Enterovirus Multiplex PCR.

Turn Around Time

1 month

Specimen Requirements

Specimens in DMEM, EMEM, Sterile PBS, Sterile saline, UTM, or VTM:  Bronchial wash, Bronchoalveolar lavage/BAL, Dual NP/OP swab, Nasal aspirate, Nasal swab, Nasal wash, Nasopharyngeal swab, Oropharyngeal swab, Sputum, Tracheal aspirate, or Vesicular fluid/skin lesion swabs

Specimens submitted in sterile container: Cerebrospinal fluid/CSF, Dry swabs of vesicular fluid/skin lesions, Skin lesions or scabs, or Stool (Raw or in Cary-Blair)

Isolates: Viral culture isolates

Preferred volume: 3 mL
Minimum volume: 200 µL

Special Considerations

Samples positive for enterovirus on the Rhinovirus and Enterovirus Multiplex PCR are reflexed to this test for characterization.

Transport/Stability

Store and ship stool, respiratory specimens, and vesicle swabs/scabs in VTM/UTM at refrigeration (2°C to 8°C) temperature within 7 days of collection. .

Store and ship cerebrospinal fluid/CSF at refrigeration (2°C to 8°C) temperature within 10 days of collection. Alternatively, ship frozen (< 0°C).

Store and ship dry samples at ambient (8°C to 25°C) temperature.

Interface/Codes

PLT: 2396

Test Resource ID
894

Ebola virus PCR

Enviado por StewaD1 el
Aliases
Ebola Zaire
Test Name
Ebola virus PCR
Test Code
LRNEBOVPCR
Methodology
Real-time PCR
Overview/Supplementary
This test detects ebola Zaire. Specimens may not be tested unless CDC has been consulted and given permission to test.
Turn Around Time
1-3 days
Specimen Requirements

Specimens: 
Paired serum (in primary collection container) and EDTA whole blood (in lavender top screw cap tube) with the same collection date and time.

Serum 
Preferred volume: 1 mL 
Minimum volume: 200 µL 

EDTA whole blood
Preferred volume: 4 mL
Minimum volume: 1 mL 

If possible, a second tube of EDTA whole blood  in a lavender top screw cap tube should be collected and submitted to forward to CDC for additional testing. 

Special Considerations

Contact Infectious Disease Epidemiology Prevention and Control (IDEPC) at 651-201-5414 or 877-676-5414 before requesting this test.

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html).

Transport/Stability
Call IDL emergency response on-call phone 612-282-3723 for shipping conditions and additional specimen submission requirements.
Interface/Codes
Test Resource ID
893

EAEC (Enteroaggregative Escherichia coli) PCR Panel

Enviado por StewaD1 el
Aliases
Test Name
EAEC (Enteroaggregative Escherichia coli) PCR Panel
Test Code

EAE

Methodology

Real-time PCR

Overview/Supplementary

This test detects enteroaggregative Escherichia coli (EAEC) from clinical specimens, using conventional culture and real-time PCR methods.

Turn Around Time

7-14 days

Specimen Requirements

Specimens: Stool or rectal swabs preserved in Para-Pak C&S, modified Cary-Blair or equivalent transport media. 

Minimum volumes: 
Rectal swab: shows visible trace of fecal material and is immersed in transport media.
Stool specimen: transport media vial filled with approximately 5 ml of stool. 

Special Considerations

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html).

Transport/Stability

Store at ambient (8°C to 25°C) temperature. Ship at ambient (8°C to 25°C) or refrigeration (2°C to 8°C) temperature.

Interface/Codes
Test Resource ID
892

Cyclospora PCR

Enviado por StewaD1 el
Aliases

Cyclo

Test Name
Cyclospora PCR
Test Code

CYCPCR

Methodology

Real-time PCR

Overview/Supplementary

This test detects Cyclospora spp. from clinical specimens. Positive samples are reflexed to additional molecular characterization for surveillance purposes.

Turn Around Time

Not Applicable

Specimen Requirements

Specimens: Stool preserved in Para-Pak C&S, modified Cary-Blair or equivalent transport media.

Special Considerations

For surveillance use only. Results not reported back to submitter.

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html).

Transport/Stability

Store and ship at ambient (8°C to 25°C) or refrigeration (2°C to 8°C) temperature.

Interface/Codes
Test Resource ID
891

Cryptosporidium Speciation and Genotyping

Enviado por StewaD1 el
Aliases

Crypto

Test Name
Cryptosporidium Speciation and Genotyping
Test Code

CRYPPCR

Methodology

Conventional PCR-RFLP, Real-time PCR, Sanger sequencing

Overview/Supplementary

This test identifies and genotypes Cryptosporidium spp. from clinical specimens.

Testing is performed for surveillance purposes and epidemiological investigations.

Turn Around Time

Not Applicable

Specimen Requirements

Specimens: Stool preserved in Para-Pak C&S, modified Cary-Blair or equivalent transport media.

Special Considerations

For surveillance use only. Results not reported back to submitter.

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html).

Transport/Stability

Store and ship at ambient (8°C to 25°C) or refrigeration (2°C to 8°C) temperature.

Interface/Codes
Test Resource ID
890

Coxiella burnetii PCR

Enviado por StewaD1 el
Aliases

Q fever

Test Name
Coxiella burnetii PCR
Test Code

COXRO

Methodology

Real-time PCR

Overview/Supplementary

This test detects Coxiella burnetii. Results from this assay are presumptive only.

Turn Around Time

1-3 days

Specimen Requirements

Nucleic Acid.

For additional requirements and specimen types, call the IDL emergency response on-call phone 612-282-3723.

Special Considerations

Call IDL emergency response on-call phone 612-282-3723 before ordering this test.

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html).

Transport/Stability

Store and ship at ambient (8°C to 25°C) or refrigeration (2°C to 8°C) temperature.

Interface/Codes

LOINC: 23024-3

Test Resource ID
888

Corynebacterium diphtheriae ID Confirmation - Isolate

Enviado por StewaD1 el
Aliases

C. diphtheriae, Diphtheria

Test Name
Corynebacterium diphtheriae ID Confirmation - Isolate
Test Code

CDIPID

Methodology

MALDI-TOF (matrix-assisted laser desorption ionization-time of flight) mass spectrometry

Overview/Supplementary

This test identifies Corynebacterium diphtheriae in submitted isolates.

When applicable, isolates will be referred for toxin testing performed at the Centers for Disease Control and Prevention laboratories, Atlanta, GA.

Turn Around Time

1-3 days: Isolate identification

2 weeks: Toxin testing

Specimen Requirements

Isolates: Actively growing culture on media that support the growth of the bacterial isolate submitted.

Special Considerations

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html).

Transport/Stability

Store and ship at ambient (8°C to 25°C) or refrigeration (2°C to 8°C) temperature.

Interface/Codes

LOINC: 42803-7

Test Resource ID
887