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Plasmodium PCR - Blood

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Aliases

Malaria, Plasmodium falciparum, Plasmodium malariae, Plasmodium ovale, Plasmodium vivax

Test Name
Plasmodium PCR - Blood
Test Code

MALPCR

Methodology

Blood parasite smear, Giemsa stain, Real-time PCR

Overview/Supplementary

This test detects Plasmodium spp. and speciates P. falciparium, P. malariae, P. ovale, and P. vivax. A blood parasite smear, Giemsa stain, will also be performed.

Turn Around Time

1-3 days

Specimen Requirements

Specimens: EDTA whole blood, Thick and thin blood film smears from whole blood

Preferred volume: 1 mL
Minimum volume: 200 µL

Special Considerations

Do not allow EDTA tube to sit for prolonged time with cap off at room temperature. Parasite and red blood cell morphology can become distorted.

Transport/Stability

Store and ship at ambient (8°C to 25°C) or refrigeration (2°C to 8°C) temperature.

Interface/Codes

LOINC: 47260-5

Test Resource ID
928

Norovirus GI/GII PCR

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Aliases

Noro

Test Name
Norovirus GI/GII PCR
Test Code

NOVPCR

Methodology

Real-time multiplex PCR

Overview/Supplementary

This test detects norovirus Genogroup 1 and Genogroup 2 in clinical specimens.

Turn Around Time

1-3 days

Specimen Requirements

Specimens: Stool in enteric transport media (Cary Blair) or unpreserved stool.

Special Considerations

Infectious Disease Epidemiology Prevention and Control (IDEPC) requested test.

Transport/Stability

Store at ambient (8°C to 25°C) temperature. Ship at ambient (8°C to 25°C) or refrigeration (2°C to 8°C) temperature.

Interface/Codes
Test Resource ID
926

Norovirus Genotype and Subtype by Sequencing

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Aliases

Noro

Test Name
Norovirus Genotype and Subtype by Sequencing
Test Code

NOVIDSEQ

Methodology

Sanger sequencing

Overview/Supplementary

This test determines norovirus genotypes using Sanger Sequencing. Testing is performed for epidemiological and surveillance purposes.

Turn Around Time

7 days

Specimen Requirements

Specimens: Stool in enteric transport media (Cary Blair) or unpreserved stool.

Special Considerations

Infectious Disease Epidemiology Prevention and Control (IDEPC) requested test.

Transport/Stability

Store at ambient (8°C to 25°C) temperature. Ship at ambient (8°C to 25°C) or refrigeration (2°C to 8°C) temperature.

Interface/Codes
Test Resource ID
925

Non-Variola Orthopoxvirus PCR - Specimen

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Aliases

Monkeypox, Mpox, MPX

Test Name
Non-Variola Orthopoxvirus PCR - Specimen
Test Code

NVOPCRSPEC

Methodology

Real-time PCR

Overview/Supplementary

This test detects non-variola orthopoxvirus (mpox) in clinical specimens.

Submission should include information about whether specimen is associated with the 2022-23 mpox outbreak. If not, please order the Variola Virus and Orthopoxvirus Rule-out (LRNPOXRO). Positive samples may be sent to CDC for additional testing.

Turn Around Time

1-7 days

Specimen Requirements

Specimens: Dry swab of lesion. Lesion crusts or scabs are acceptable, but not recommended, and must be submitted with a swab from the same lesion.

Collect samples from more than one lesion, preferably from different locations on the body and/or from lesions with differing appearances.

Specimen collection:

Collect two swabs from each lesion. Vigorously swab or brush lesion with two separate sterile dry nylon, polyester, or Dacron swabs. Either break off the applicator end of both swabs into the same 1.5mL or 2mL screw-capped tube with an O-ring (preferred) or place two swabs from one lesion into one sterile container. Use separate sterile containers for each set of same lesion swabs.

Refrigerate within 1 hour of collection.

No cotton swabs or swabs received in transport media will be accepted.

Special Considerations

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html).

Transport/Stability

Ship at refrigeration (2°C to 8°C) temperature. Specimens must received be within 7 days of collection.

Interface/Codes
Test Resource ID
924

Neonatal Sepsis - Isolate Submission

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Aliases

Sepsis

Test Name
Neonatal Sepsis - Isolate Submission
Test Code

NEOSEP

Methodology

16S Sanger sequencing, Biochemicals, MALDI-TOF (matrix-assisted laser desorption ionization-time of flight) mass spectrometry

Overview/Supplementary

This test confirms the identification of organisms causing neonatal sepsis and is for the surveillance of neonatal sepsis. Submit all bacteria, excluding coagulase-negative Staphylococcus, isolated from a sterile site in infants less than 7 days of age.

If submitting Haemophilus influenzae, Neisseria meningitidis, MRSA, MSSA, Group A Streptococcus, Group B Streptococcus, or Streptococcus pneumoniae, see organism specific tests.

Turn Around Time

Not Applicable

Specimen Requirements

Isolates: Actively growing culture on media that support the growth of the bacterial isolate submitted.

Special Considerations

For surveillance use only. Results not reported back to submitter.

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html).

Transport/Stability

Store and ship at ambient (8°C to 25°C) or refrigeration (2°C to 8°C) temperature.

Interface/Codes

LOINC: 43409-2

Test Resource ID
923

Neisseria meningitidis PCR - Specimen

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Aliases

Meningitis, N. meningitidis

Test Name
Neisseria meningitidis PCR - Specimen
Test Code

NMENPCRS

Methodology

Real-time PCR

Overview/Supplementary

This test detects Neisseria meningitidis in specimens. If N. meningitidis is detected, serotyping is performed for serotypes A, B, C, W135, X, and Y.

Turn Around Time

2-5 days

Specimen Requirements

Specimens: Cerebrospinal fluid/CSF (preferred), EDTA whole blood.

Preferred volume: 200 µL

Special Considerations

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html).

Transport/Stability

Store and ship at refrigeration (2°C to 8°C) or frozen (≤0°C) temperature within 7 days of collection.

Interface/Codes

PLT: 2406

Test Resource ID
922

Neisseria meningitidis Serotyping PCR - Isolate

Enviado por StewaD1 el
Aliases

Meningitis, N. meningitidis

Test Name

Neisseria meningitidis Serotyping PCR - Isolate

Test Code

NMENSERO

Methodology

Real-time PCR

Overview/Supplementary

This test identifies Neisseria meningitidis in isolates. If N. meningitidis is detected, serotyping is performed for serotypes A, B, C, W135, X, and Y.

Turn Around Time

2-5 days

Specimen Requirements

Isolates: Actively growing culture on media that support the growth of the bacterial isolate submitted. (e.g., Blood agar, Chocolate agar).

Use slant with screw cap or ensure plates are sealed.

Special Considerations

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html).

Transport/Stability

Store and ship at ambient (8°C to 25°C) or refrigerated (2°C to 8°C) temperature.

Interface/Codes

PLT: 2406

Test Resource ID
921

Neisseria gonorrhoeae - Isolate

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Aliases

GC, Gonorrhea

Test Name
Neisseria gonorrhoeae - Isolate
Test Code

NGONID

Methodology

MALDI-TOF (matrix-assisted laser desorption ionization-time of flight) mass spectrometry

Overview/Supplementary

This test confirms the identification of Neisseria gonorrhoeae from isolates collected from invasive specimens. This test is for surveillance of disseminated gonococcal infection.

Turn Around Time

1-3 days

Specimen Requirements

Isolates: Actively growing culture from an invasive site on media that support the growth of the bacterial isolate submitted.

Special Considerations

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html)

Transport/Stability

Store and ship at ambient (8°C to 25°C) or refrigerated (2°C to 8°C) temperature.

Interface/Codes

LOINC: 698-1

Test Resource ID
919

Neisseria gonorrhoeae Culture - Specimen

Enviado por StewaD1 el
Aliases

GC, GISP, N. gonorrhoeae

Test Name
Neisseria gonorrhoeae Culture - Specimen
Test Code

NGONCU

Methodology

Culture, MALDI-TOF (matrix-assisted laser desorption ionization-time of flight) mass spectrometry

Overview/Supplementary

This test identifies Neisseria gonorrhoeae from clinical specimens. The test is for select clinics who participate in the Gonococcal Isolate Surveillance Program (GISP) in collaboration with MDH-PHL and the Centers for Disease Control and Prevention.

Turn Around Time

5-7 days

Specimen Requirements

Specimens: Urethral swabs plated to modified Thayer-Martin (MTM) at the bedside of the submitting facility or clinic.

Special Considerations

None

Transport/Stability

Plates should be incubated in a candle jar or with 4-6% CO₂ at 35-37°C until transport.

Ship at ambient (8°C to 25°C) temperature.

Interface/Codes

LOINC: 698-1

Test Resource ID
920

Mycobacterium tuberculosis Isolate Submission

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Aliases

Acid-fast bacilli, AFB, MTB, MTBC, Mycobacterium, TB, Tuberculosis

Test Name
Mycobacterium tuberculosis Isolate Submission
Test Code

MTBISO

Methodology

Kinyoun carbol fuchsin stain

Overview/Supplementary

This test confirms the identification of Mycobacterium tuberculosis Complex from isolates.

Turn Around Time

Up to 3 weeks

Specimen Requirements

Isolates: Actively growing culture on media that support the growth of the bacterial isolate submitted (e.g., MGIT broth, MP broth, or L7H11 agar).

Special Considerations

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html).

Transport/Stability

Store and ship at ambient (8°C to 25°C) or refrigerated (2°C to 8°C) temperature.

Interface/Codes
Test Resource ID
918