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Mycobacterium Culture and Acid-fast Smear

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Aliases
Acid-fast bacilli, AFB, MAC, MTB, MTBC, Mycobacterium, TB, Tuberculosis
Test Name
Mycobacterium Culture and Acid-fast Smear
Test Code
AFBCU
Methodology
16S Sanger sequencing, Auramine-rhodamine fluorochrome smear, BACTEC™MGIT™ 960 Mycobacteria culture system,INNO-LiPA® line probe assay
Overview/Supplementary
This test detects mycobacteria in clinical specimens. For isolates, see Acid-fast Bacillus Identification - Isolate.
Turn Around Time

1 day: Smear result

6 weeks of incubation: Negative culture reported

Specimen Requirements

Refer to Acid-Fast Bacilli Specimen Collection and Transport Criteria (PDF) for specimen requirements.

Special Considerations
None
Transport/Stability

Refer to Acid-Fast Bacilli Specimen Collection and Transport Criteria (PDF) for specimen requirements.

Interface/Codes
LOINC: 40699-1
Test Resource ID
917

Mumps Virus PCR

Enviado por StewaD1 el
Aliases

Parotid swelling

Test Name
Mumps Virus PCR
Test Code

MUVPCR

Methodology

Real-time PCR

Overview/Supplementary

This test detects mumps by real-time PCR from specimens or viral culture isolates. Respiratory Virus Isolation and Identification (MMRCULT) will be performed on negative respiratory specimens.

Turn Around Time

1-3 days

Specimen Requirements

Specimens in Liquid Stuarts, M4, M5, Sterile PBS, Sterile saline, UTM, or VTM: Buccal swab (preferred source, up to 9 days post onset), Nasal swab, Nasal wash, Nasopharyngeal swab, Nasopharyngeal wash, or Throat swab

Specimens in a sterile container: Cerebrospinal fluid/CSF (if meningitis or encephalitis suspected), or Urine  (if collected 5 or more days post onset, pair with buccal swab)

Isolates: Viral culture isolate

Preferred volume: 3 mL
Minimum volume: 140 µL

Special Considerations

Contact Infectious Disease Epidemiology Prevention and Control (IDEPC) at 651-201-5414 or 877-676-5414 before requesting this test.

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html).

Transport/Stability

Store and ship at refrigeration (2°C to 8°C) temperature within 7 days of collection. Alternatively, ship frozen (≤0°C) within 1 year.

Interface/Codes

LOINC: 47532-7

Test Resource ID
916

M. tuberculosis Complex and M. avium Complex PCR

Enviado por StewaD1 el
Aliases

Acid-fast bacilli, AFB, MAC, MTB, MTBC, Mycobacterium, TB, Tuberculosis

Test Name
M. tuberculosis Complex and M. avium Complex PCR
Test Code

MTBCPCR

Methodology

Real-time PCR

Overview/Supplementary

This test detects M. tuberculosis Complex and M. avium Complex.

Turn Around Time

1-3 days

Specimen Requirements

Specimens: Sputum (preferred source), Bronchial wash, Bronchoalveolar lavage/BAL, NaOH/NALC digested specimen

Preferred volume: 5.0 mL
Minimum volume: 0.5 mL

Special Considerations

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html).

Transport/Stability

Store and ship at refrigeration (2°C to 8°C) temperature within 7 days of collection.

Interface/Codes
Test Resource ID
911

MSSA Isolate Submission

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Aliases

Methicillin-susceptible Staphylococcus aureus

Test Name
MSSA Isolate Submission
Test Code

MSSAID

Methodology

MALDI-TOF (matrix-assisted laser desorption ionization-time of flight) mass spectrometry

Overview/Supplementary

This test confirms the identification of methicillin-susceptible Staphylococcus aureus (MSSA) from isolates collected from invasive specimens. This test is for surveillance of invasive MSSA from patients who reside in Hennepin or Ramsey counties.

Turn Around Time

Not Applicable

Specimen Requirements

Isolates: Actively growing culture from an invasive site on media that support the growth of the bacterial isolate submitted.

Special Considerations

For surveillance use only. Results not reported back to submitter.

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html).

Transport/Stability

Store and ship at ambient (8°C to 25°C) or refrigeration (2°C to 8°C) temperature.

Interface/Codes

LOINC: 43409-2

Test Resource ID
915

MRSA Isolate Submission

Enviado por StewaD1 el
Aliases

Methicillin-resistant Staphylococcus aureus

Test Name
MRSA Isolate Submission
Test Code

MRSAID

Methodology

MALDI-TOF (matrix-assisted laser desorption ionization-time of flight) mass spectrometry

Overview/Supplementary

This test confirms the identification of methicillin-resistant Staphylococcus aureus (MRSA) from isolates collected from invasive specimens. This test is for surveillance of invasive MRSA from patients who reside in Hennepin or Ramsey counties.

Turn Around Time

Not Applicable

Specimen Requirements

Isolates: Actively growing culture from an invasive site on media that support the growth of the bacterial isolate submitted.

Special Considerations

For surveillance use only. Results not reported back to submitter.

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html).

Transport/Stability

Store and ship at ambient (8°C to 25°C) or refrigeration (2°C to 8°C) temperature.

Interface/Codes

LOINC: 43409-2

Test Resource ID
914

Measles Virus PCR

Enviado por StewaD1 el
Aliases

Rubeola

Test Name

Measles Virus PCR

Test Code

MEVPCR

Methodology

Real-time PCR

Overview/Supplementary

This test detects measles virus in clinical specimens. Respiratory Virus Isolation and Identification (MMRCULT) will be performed on negative respiratory specimens.

Turn Around Time

1-3 days

Specimen Requirements

Specimens in VTM (preferred medium), Balanced Salt Solution (BSS), Liquid Stuart's, M4, M5, Minimum essential medium (MEM), Sterile isotonic solutions, Sterile PBS, Sterile saline, or UTM: Throat swab (preferred source, up to 9 days post onset), Nasal swab, Nasal wash, Nasopharyngeal swab, or Nasopharyngeal wash 

Specimens in a sterile container: Cerebrospinal fluid/CSF (if meningitis or encephalitis suspected), or Urine (if collected 5 or more days post onset, pair with throat swab) 

Isolates: Viral culture isolate 

Preferred volume: 3 mL 

Minimum volume: 140 µL

Special Considerations

Please include vaccination status for MMR with submission. 

Contact Infectious Disease Epidemiology Prevention and Control at 651-201-5414 or 877-676-5414 for further information when requesting this test. 

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html).

Transport/Stability

Store and ship at refrigeration (2°C to 8°C) temperature within 7 days of collection. Alternatively, ship frozen (≤0°C).

Interface/Codes

LOINC: 85806-8

Test Resource ID
913

Measles Vaccine Strain PCR

Enviado por StewaD1 el
Aliases

Rubeola, Vaccine typing

Test Name

Measles Vaccine Strain PCR

Test Code

MEVAPCR

Methodology

Real-time PCR

Overview/Supplementary

This test detects the vaccine strain of measles virus in clinical samples in patients who have received recent measles vaccination.

Turn Around Time

1-3 days

Specimen Requirements

Specimens in VTM (preferred medium), Balanced Salt Solution (BSS), Liquid Stuart's, M4, M5, Minimum essential medium (MEM), Sterile isotonic solutions, Sterile PBS, Sterile saline, or UTM: Throat swab (preferred source, up to 9 days post onset), Nasal swab, Nasal wash, Nasopharyngeal swab, or Nasopharyngeal wash 

Specimens in a sterile container: Cerebrospinal fluid/CSF (if meningitis or encephalitis suspected), or Urine (if collected 5 or more days post onset, pair with throat swab) 

Isolates: Viral culture isolate 

Preferred volume: 3 mL 

Minimum volume: 140 µL

Special Considerations

This test should only be requested if there is recent measles vaccination within 21 days of rash onset.

Vaccination history must be provided to request this test.

Contact Infectious Disease Epidemiology Prevention and Control at 651-201-5414 or 877-676-5414 for further information when requesting this test.

Transport/Stability

Store and ship at refrigeration (2°C to 8°C) temperature within 7 days of collection. Alternatively, ship frozen (≤0°C).

Interface/Codes
Test Resource ID
912

Listeria Identification - Isolate

Enviado por StewaD1 el
Aliases

L. monocytogenes, Listeria monocytogenes

Test Name
Listeria Identification - Isolate
Test Code

LISTID

Methodology

Biochemicals, MALDI-TOF (matrix-assisted laser desorption ionization-time of flight) mass spectrometry

Overview/Supplementary

This test confirms the identification of Listeria spp. in submitted isolates.

Turn Around Time

1-3 days

Specimen Requirements

Isolates: Actively growing culture on media that support the growth of the bacterial isolate submitted.

Special Considerations

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html).

Transport/Stability

Store and ship at ambient (8°C to 25°C) or refrigeration (2°C to 8°C) temperature.

Interface/Codes

LOINC: 6609-2

Test Resource ID
910

Legionella Identification - Isolate

Enviado por StewaD1 el
Aliases

L. pneumophila, Legionella pneumophila

Test Name
Legionella Identification - Isolate
Test Code

LEGID

Methodology

16S Sanger sequencing, Fluorescent antibody stain, MALDI-TOF (matrix-assisted laser desorption ionization-time of flight) mass spectrometry, Real-time PCR

Overview/Supplementary

This test confirms the identification of Legionella spp. in submitted isolates. Serogrouping is performed on confirmed isolates.

Turn Around Time

1-3 days

Specimen Requirements

Isolates: Actively growing culture on media that support the growth of the bacterial isolate submitted (e.g., Buffered charcoal yeast extract agar).

Special Considerations

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html).

Transport/Stability

Store and ship at ambient (8°C to 25°C) or refrigeration (2°C to 8°C) temperature.

Interface/Codes

LOINC: 593-4

Test Resource ID
909

Legionella Culture - Specimen

Enviado por StewaD1 el
Aliases

L. pneumophila, Legionella pneumophila

Test Name
Legionella Culture - Specimen
Test Code

LEGCU

Methodology

16S Sanger sequencing, Culture, Fluorescent antibody stain, MALDI-TOF (matrix-assisted laser desorption ionization-time of flight) mass spectrometry, Real-time PCR

Overview/Supplementary

This test isolates, detects, and identifies Legionella spp. from clinical specimens for diagnostic and surveillance purposes.

Turn Around Time

Up to 14 days

Specimen Requirements

Specimens: Sputum, Blood, Bone marrow, Bronchial washings, Bronchial aspirates, Bronchoalveolar lavage/BAL, Lung tissue,    Organ autopsy material, Pericardial fluid, Peritoneal fluid, sinuses, Pleural fluid,  Tracheal aspirates.

Preferred volume: ≥ 1mL.
Minimum volume: 200 µL.

Urine is NOT acceptable. 

Special Considerations

If submitting specimens due to a positive Legionella urinary antigen test or molecular assay, enter Project # 2126 on the submission form.

For environmental samples, see Legionella species PCR - Specimen.

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html),

Transport/Stability

Store and ship at refrigeration (2°C to 8°C) temperature. Alternatively, freeze (≤0°C) and ship on dry ice.

Interface/Codes

LOINC: 593-4

Test Resource ID
908