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Corynebacterium diphtheriae Culture - Specimen

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Aliases

C. diphtheriae culture, Diphtheria

Test Name
Corynebacterium diphtheriae Culture - Specimen
Test Code

CDIPCU

Methodology

Biochemicals, Culture, MALDI-TOF (matrix-assisted laser desorption ionization-time of flight) mass spectrometry

Overview/Supplementary

This test isolates and identifies Corynebacterium diphtheriae from clinical specimens.

If Corynebacterium diphtheriae is identified, the isolate will be referred for toxin testing to the Centers for Disease Control and Prevention laboratories, Atlanta, GA.

Turn Around Time

3-7 days: Culture

2 weeks: Toxin testing

Specimen Requirements

Specimens: Nasopharynx, throat, or wound swab transported in Amies or Stuart media.

If possible, collect from several areas to increase sensitivity.

If cutaneous diphtheria is suspected, it is recommended to collect skin, throat, and nasopharynx swabs.

Special Considerations

Requests for Diphtheria culture and antitoxin should be called to the Infectious Disease Epidemiology, Prevention, and Control Department (651-201-5414).

Notify the MDH microbiology laboratory prior to specimen collection so that Tinsdale agar can be prepared.

Transport/Stability

Transport specimen at refrigerated (2°C to 8°C) temperature on ice within 24 hours of collection.

If there is a delay in shipping, place inoculated swab directly into Loeffler's media slants and incubate at 35 ± 2°C until shipped, then ship at ambient (8°C to 25°C) temperature.

Interface/Codes

LOINC: 16676-9

Test Resource ID
886

Coccidioides PCR - Isolate

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Aliases

Coccidioides immitis, Coccidioides posadasii, Fungal PCR

Test Name
Coccidioides PCR - Isolate
Test Code

COCCIPCR

Methodology

Real-time PCR

Overview/Supplementary

This test detects Coccidioides immitis and Coccidioides posadasii but does not differentiate the two. MDH will decide if additional tests are required, including fungal identification.

Turn Around Time

1-3 days

Specimen Requirements

Isolates: Actively growing culture on media that support the growth of the fungal isolate submitted.

Use slant with screw cap or ensure plates are sealed.

Special Considerations

None

Transport/Stability

Store and ship at ambient (8°C to 25°C) or refrigerated (2°C to 8°C) temperature.

Interface/Codes

LOINC: 97916-1

Test Resource ID
885

Clostridium botulinum Rule-out

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Aliases
Botulism, Botulinum toxin, Cbot
Test Name
Clostridium botulinum Rule-out
Test Code
CBOTRO
Methodology
Culture, Functional toxin assay (mouse bioassay)
Overview/Supplementary
This test detects Clostridium botulinum from clinical specimens using a mouse bioassay. Real-time PCR may also be performed to help target the toxin type for the Mouse Bioassay. PCR results will not be reported.
Turn Around Time
7-14 days
Specimen Requirements

Specimens: Call IDL emergency response on-call phone 612-282-3723 for requirements for specimen submission.

Refer to Botulism Testing and Shipping Requirements (PDF) for specimen requirements.

Special Considerations

Contact Infectious Disease Epidemiology Prevention and Control (IDEPC) at 651-201-5414 or 877-676-5414 before requesting this test.

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html).

Transport/Stability
Call IDL emergency response on-call phone 612-282-3723 for shipping conditions and additional specimen submission requirements.
Interface/Codes
LOINC: 33696-6
Test Resource ID
884

Carbapenemase-producing Organism Culture

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Aliases

Carbapenem-resistant organisms, CPO, CP-CRA, CP-CRAB, CP-CRE, CP-CRPA, CRA, CRAB, CRE, CRPA, CRO, KPC, NDM, VIM, IMP, OXA-23, OXA-24, OXA-48, OXA-58

Test Name
Carbapenemase-producing Organism Culture
Test Code

CPOCX

Methodology

Biochemicals, Conventional PCR, Culture, MALDI-TOF (matrix-assisted laser desorption ionization-time of flight) mass spectrometry, Real-time PCR

Overview/Supplementary

This test detects KPC, NDM, VIM, IMP, OXA-23, OXA-24, OXA-48, and OXA-58 gene sequences associated with carbapenem-resistant organisms (CRO).

CRO testing is used as an aid in infection control in the detection of CRO that colonize patients in health care settings and is not intended to guide or monitor treatment for CRO bacterial infections.

Turn Around Time

3-7 days

Specimen Requirements

Specimens: Rectal source (Copan dual swab collection kit or equivalent), Wound or skin axilla and groin composite source (Eswab), Sputum, Stool.

Special Considerations

If submitting specimens for a point prevalence survey, notify MDH-PHL via email arlnmn@state.mn.us.

Fill out ARLAB Central Region Clinical Testing and Submission form https://www.health.state.mn.us/diseases/idlab/arln.html.

Transport/Stability

Store and ship at ambient (8°C to 25°C) or refrigerated (2°C to 8°C) temperature.

If Carbapenemase PCR, CarbaR - Specimen testing is also ordered on the same rectal swab, store and ship at ambient (8°C to 25°C) temperature within 5 days of collection.

Interface/Codes

LOINC: 73834-4

Test Resource ID
883

Carbapenemase PCR CarbaR - Specimen

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Aliases

Carbapenem-resistant organisms, CPO, CP-CRA, CP-CRAB, CP-CRE, CP-CRPA, CRA, CRAB, CRE, CRPA, CRO, KPC, NDM, VIM, IMP, OXA-48

Test Name

Carbapenemase PCR CarbaR - Specimen

Test Code

CARBAR

Methodology

Real-time PCR

Overview/Supplementary

This test detects KPC, NDM, VIM, IMP, and OXA-48 gene sequences associated with carbapenem-resistant organisms (CRO) in clinical specimens.

CRO testing is used as an aid in infection control in the detection of CRO that colonize patients in health care settings and is not intended to guide or monitor treatment for CRO bacterial infections.

Turn Around Time

1-3 days

Specimen Requirements

Specimens: Rectal or peri-rectal swab collected using Copan dual swab collection kit, or equivalent, received within 5 days of collection.

Special Considerations

If submitting specimens for a point prevalence survey, notify MDH-PHL via email arlnmn@state.mn.us.

Fill out ARLAB Central Region Clinical Testing and Submission form https://www.health.state.mn.us/diseases/idlab/arln.html.

Transport/Stability

Store and ship at ambient (15°C to 28°C) temperature within 5 days of collection.

Interface/Codes

LOINC: 85502-3

Test Resource ID
882

Carbapenemase Detection in Pseudomonas - Isolate

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Aliases

Carbapenem-resistant Pseudomonas, CP-CRPA, CRPA, KPC, NDM, VIM, IMP, and OXA-48

Test Name
Carbapenemase Detection in Pseudomonas - Isolate
Test Code

CRP

Methodology

16S Sanger sequencing, Biochemicals, Carba NP, Conventional PCR, MALDI-TOF (matrix-assisted laser desorption ionization-time of flight) mass spectrometry, Modified carbapenem inactivation method (mCIM), Real-time PCR

Overview/Supplementary

This test detects the following carbapenemase genes in Pseudomonas spp.: KPC, NDM, VIM, IMP, and OXA-48.

Turn Around Time

2-7 days

Specimen Requirements

Isolates: Actively growing culture on media that support the growth of the bacterial isolate submitted.

Isolates should be subcultured as few times as possible prior to submitting for testing to limit the potential for resistance plasmid loss during passage.

If a culture isolate(s) is submitted for testing, the original culture/isolate/inoculum should be maintained by the submitter until MDH results are reported.

Special Considerations

Submit copy of antimicrobial susceptibility testing results from clinical lab along with the isolate.If submitting isolate for AR Lab Network, please fill out ARLAB Central Region Clinical Testing and Submission form https://www.health.state.mn.us/diseases/idlab/arln.html.

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html).

Transport/Stability

Store and ship at ambient (8°C to 25°C) or refrigeration (2°C to 8°C) temperature.

Interface/Codes

LOINC: 86930-5

Test Resource ID
881

Carbapenemase Detection in Enterobacterales - Isolate

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Aliases

Carbapenem-resistant Enterobacterales, CP-CRE, CRE, KPC, NDM, VIM, IMP, OXA-48

Test Name
Carbapenemase Detection in Enterobacterales - Isolate
Test Code

CRE

Methodology

16S Sanger sequencing, Biochemicals, Carba NP, Conventional PCR, MALDI-TOF (matrix-assisted laser desorption ionization-time of flight) mass spectrometry, Modified carbapenem inactivation method (mCIM), Real-time PCR

Overview/Supplementary

This test detects the following carbapenemase genes in Enterobacterales: KPC, NDM, VIM, IMP, and OXA-48.

Turn Around Time

2-7 days

Specimen Requirements

Isolates: Actively growing culture on media that support the growth of the bacterial isolate submitted.

Isolates should be subcultured as few times as possible prior to submitting for testing to limit the potential for resistance plasmid loss during passage.

If a culture isolate(s) is submitted for testing, the original culture/isolate/inoculum should be maintained by the submitter until MDH results are reported.

Special Considerations

Submit copy of antimicrobial susceptibility testing results from clinical lab along with the isolate.

If submitting isolate for AR Lab Network, please fill out ARLAB Central Region Clinical Testing and Submission form https://www.health.state.mn.us/diseases/idlab/arln.html.

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html)

Transport/Stability

Store and ship at ambient (8°C to 25°C) or refrigeration (2°C to 8°C) temperature.

Interface/Codes

LOINC: 86930-5

Test Resource ID
880

Carbapenemase Detection in Acinetobacter - Isolate

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Aliases
A. baumannii, Carbapenem-resistant Acinetobacter, CP-CRA, CP-CRAB, CRA, CRAB, KPC, NDM, VIM, IMP, OXA-23, OXA-24, OXA-48, OXA-58
Test Name
Carbapenemase Detection in Acinetobacter - Isolate
Test Code
CRA
Methodology
MALDI-TOF (matrix-assisted laser desorption ionization-time of flight) mass spectrometry, Real-time PCR
Overview/Supplementary
This test detects the following carbapenemase genes in Acinetobacter sp.: KPC, NDM, VIM, IMP, OXA-23, OXA-24, OXA-48, and OXA-58.
Turn Around Time
2-7 days
Specimen Requirements

Isolates: Actively growing culture on media that support the growth of the bacterial isolate submitted. 

Isolates should be subcultured as few times as possible prior to submitting for testing to limit the potential for resistance plasmid loss during passage. 

If a culture isolate(s) is submitted for testing, the original culture/isolate/inoculum should be maintained by the submitter until MDH results are reported.

Special Considerations

Submit copy of antimicrobial susceptibility testing results from clinical lab along with the isolate.

If submitting isolate for AR Lab Network, please fill out ARLAB Central Region Clinical Testing and Submission form https://www.health.state.mn.us/diseases/idlab/arln.html.

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html)

Transport/Stability

Store and ship at ambient (8°C to 25°C) or refrigeration (2°C to 8°C) temperature.

Interface/Codes
LOINC: 86930-5
Test Resource ID
879

Candida auris PCR

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Aliases

C. auris

Test Name

Candida auris PCR

Test Code

CAPCRPF

Methodology

Real-time PCR

Overview/Supplementary

This test detects the gene sequence rDNA ITS2 from C. auris directly from patient specimens.

C. auris testing is used as an aid in infection control in the detection of C. auris that colonizes patients in health care settings and is not intended to guide or monitor treatment of infection.

Turn Around Time

2-5 days

Specimen Requirements

Specimens: Skin swab (optimal axilla and groin composite) collected with an Eswab

Special Considerations

If submitting specimens for a point prevalence survey, notify MDH-PHL via email arlnmn@state.mn.us.

Fill out ARLAB Central Region Clinical Testing and Submission form https://www.health.state.mn.us/diseases/idlab/arln.html.

Transport/Stability

Store and ship at ambient (8°C to 25°C) or refrigeration (2°C to 8°C) temperature.

Interface/Codes

LOINC: 87620-1

Test Resource ID
878

Candida auris Colonization Culture

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Aliases

C. auris

Test Name

Candida auris Colonization Culture

Test Code

CACOL

Methodology

Culture, MALDI-TOF (matrix-assisted laser desorption ionization-time of flight) mass spectrometry

Overview/Supplementary

This test identifies Candida auris by salt broth culture method. This test is generally performed on specimens that test positive or indeterminate for Candida auris by PCR.

C. auris testing is used as an aid in infection control in the detection of C. auris that colonizes patients in health care settings and is not intended to guide or monitor treatment of infection.

Turn Around Time

7-9 days

Specimen Requirements

Specimens: Skin swab (optimal axilla and groin composite) collected with an Eswab

Special Considerations

Fill out ARLAB Central Region Clinical Testing and Submission form https://www.health.state.mn.us/diseases/idlab/arln.html
 

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000-4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html).

Transport/Stability

Store and ship at ambient (8°C to 25°C) or refrigeration (2°C to 8°C) temperature.

Interface/Codes

LOINC: 90002-7

Test Resource ID
877