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Vibrio Culture - Stool

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Aliases

Cholera

Test Name
Vibrio Culture - Stool
Test Code

VIBRIOCU

Methodology

Biochemicals, Culture, MALDI-TOF (matrix-assisted laser desorption ionization-time of flight) mass spectrometry, Whole genome sequencing

Overview/Supplementary

This test detects and identifies Vibrio spp. in the submitted stool specimen.

Turn Around Time

5-7 days

Specimen Requirements

Specimens: Stool or rectal swabs preserved in Para-Pak C&S, modified Cary-Blair or equivalent transport media. 

Minimum volumes: 
Rectal swab: shows visible trace of fecal material and is immersed in transport media.
Stool specimen: transport media vial filled with approximately 5 ml of stool. 

Special Considerations

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html)

Transport/Stability

Store at ambient (8°C to 25°C) temperature. Ship at ambient (8°C to 25°C) or refrigeration (2°C to 8°C) temperature.

Interface/Codes
Test Resource ID
948

Variola Virus and Orthopoxvirus Rule-Out

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Aliases

Poxvirus, Monkeypox, Mpox, Smallpox, Vaccinia

Test Name
Variola Virus and Orthopoxvirus Rule-Out
Test Code

LRNPOXRO

Methodology

Real-time PCR

Overview/Supplementary

This test detects poxviruses, including variola virus, orthopoxvirus, non-variola virus and varicella-zoster from clinical specimens. Results are presumptive only.

Specimen may be sent to CDC for further characterization.

If testing is for a specimen associated with concern for mpox (2022-23 mpox outbreak), please order Non-Variola Orthopoxvirus PCR - Specimen (NVOPCRSPEC) instead.

Turn Around Time

1-3 days

Specimen Requirements

Specimens: Dry swab of lesion, lesion crusts, or scabs

Cotton swabs or swabs received in transport media will not be accepted. 

Call IDL emergency response on-call phone 612-282-3723 for additional requirements.

Special Considerations

Contact Infectious Disease Epidemiology Prevention and Control (IDEPC) at 651-201-5414 or 877-676-5414 before requesting this test.

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html).

Transport/Stability

Ship at refrigeration (2°C to 8°C) temperature. Specimens must received be within 7 days of collection.

Interface/Codes
Test Resource ID
947

Varicella-zoster Virus PCR

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Aliases

Chickenpox, Shingles, VZV

Test Name

Varicella-zoster Virus PCR

Test Code

VZV_PCR

Methodology

Real-time PCR

Overview/Supplementary

This test distinguishes between wild-type VZV and vaccine strain.

Turn Around Time

1-3 days

Specimen Requirements

Specimens submitted dry or in UTM or VTM: Vesicular fluid swabs

Samples in sterile container: Cerebrospinal fluid/CSF, or Skin lesions or scabs (dry)

Isolates: Viral culture isolate

Preferred volume: 1 mL
Minimum volume: 200 µL 

Special Considerations

None

Transport/Stability

Store and ship dry samples at ambient (8°C to 25°C) temperature. Store and ship other specimens at refrigeration (2°C to 8°C) temperature.

Interface/Codes
Test Resource ID
946

Syphilis VDRL - CSF

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Aliases

Neurosyphilis, Treponema pallidum

Test Name
Syphilis VDRL - CSF
Test Code

SYPHVDRL

Methodology

Particle agglutination

Overview/Supplementary

This test confirms the presence of Treponema pallidium, the causative agent of syphilis, in clinical specimens.

Quantitative titer will be performed on positive specimens.

Turn Around Time

5-7 days

Specimen Requirements

Specimens: Cerebrospinal fluid/CSF 

Preferred volume: 1 mL
Minimum volume: 200 µL

Special Considerations

None

Transport/Stability

Store and ship at refrigeration (2°C to 8°C) temperature within 5 days of collection. Alternatively, ship frozen (≤0°C).

Interface/Codes

LOINC: 14904-7

Test Resource ID
945

Syphilis Screen by RPR - Serum

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Aliases

Treponema pallidum

Test Name
Syphilis Screen by RPR - Serum
Test Code

SYPHRPR

Methodology

Particle agglutination

Overview/Supplementary

This test screens for Treponema pallidium, causative agent of syphilis, in clinical specimens.

Quantitative titer will be performed on positive specimens.

Turn Around Time

3-5 days

Specimen Requirements

Specimen: Serum

Collect in a serum separator tubes (SST). Separate serum from cells within 2 hours of collection. Transfer sample to a sterile tube for transport. 

Preferred volume: 1 mL 
Minimum volume: 200 µL

Grossly hemolyzed samples will be rejected.

Special Considerations

None

Transport/Stability

Store and ship at refrigeration (2°C to 8°C) temperature within 5 days of collection. Alternatively, ship frozen (≤0°C).

Interface/Codes
Test Resource ID
944

Syphilis Confirmation by TPPA - Serum

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Aliases

Treponema pallidum

Test Name
Syphilis Confirmation by TPPA - Serum
Test Code

SYPHTPPA

Methodology

Particle agglutination

Overview/Supplementary

This test confirms the presence of Treponema pallidium, the causative agent of syphilis, in clinical specimens.

Turn Around Time

3-5 days

Specimen Requirements

Specimen: Serum

Collect in a serum separator tubes (SST). Separate serum from cells within 2 hours of collection. Transfer sample to a sterile tube for transport. 

Preferred volume: 1 mL 
Minimum volume: 200 µL

Grossly hemolyzed samples will be rejected.

Special Considerations

None

Transport/Stability

Store and ship at refrigeration (2°C to 8°C) temperature within 5 days of collection. Alternatively, ship frozen (≤0°C).

Interface/Codes

LOINC: 24312-1

Test Resource ID
943

Streptococcus Special Project

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Aliases

GAS, GBS, SNIPP, S. agalactiae, S. pneumoniae, S. pyogenes

Test Name
Streptococcus Special Project
Test Code

STREPSP

Methodology

Specimens are referred to the Centers for Disease Control and Prevention (CDC) in Atlanta, GA for testing.

Overview/Supplementary

This test identifies Streptococcus spp. from clinical specimens that were positive for S. pneumoniae, S. pyogenes (GAS), or S. agalactiae (GBS) from blood and urine specimens positive for Streptococcus pneumoniae by urinary antigen testing. Testing is performed for surveillance purposes.

Turn Around Time

Not Applicable

Specimen Requirements

Specimens: 

Blood (whole blood from blood culture bottle)
Preferred volume: 10 mL
Minimum volume: 2 mL

Cerebrospinal fluid/CSF
Preferred volume: 2 mL
Minimum volume: 0.2 mL

Urine
Minimum volume: 2 mL

Special Considerations

For surveillance use only. Results not reported back to submitter.

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html).

Transport/Stability

Store and ship at refrigeration (2°C to 8°C) temperature.

Interface/Codes
Test Resource ID
942

Streptococcus pneumoniae Serotyping - Isolate

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Aliases

Strep pneumo

Test Name
Streptococcus pneumoniae Serotyping - Isolate
Test Code

SPNSER

Methodology

Whole genome sequencing

Overview/Supplementary

This test confirms the identification and serotypes Streptococcus pneumoniae from isolates collected from invasive specimens.

Turn Around Time

7-14 days

Specimen Requirements

Isolates: Actively growing culture from an invasive site on media that support the growth of the bacterial isolate submitted.

Special Considerations

If submitting isolate for AR Lab Network, please fill out ARLAB Central Region Clinical Testing and Submission form: https://www.health.state.mn.us/diseases/idlab/arln.html.

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html).

Transport/Stability

Store and ship at ambient (8°C to 25°C) or refrigerated (2°C to 8°C) temperature.

Interface/Codes

LOINC: 85688-0

Test Resource ID
941

Streptococcus pneumoniae PCR - Specimen

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Aliases

Meningitis, S. pneumoniae

Test Name
Streptococcus pneumoniae PCR - Specimen
Test Code

SPNPCRS

Methodology

Real-time PCR

Overview/Supplementary

This test detects Streptococcus pneumoniae in clinical specimens. If S. pneumoniae is detected, serotyping is performed.

Turn Around Time

2-5 days

Specimen Requirements

Specimens: Cerebrospinal fluid/CSF (preferred), EDTA whole blood

Preferred volume: 200 µL

Special Considerations

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html).

Transport/Stability

Store and ship at refrigeration (2°C to 8°C) or frozen (≤0°C) temperature within 7 days of collection.

Interface/Codes

PLT: 2716

Test Resource ID
940

Streptococcus (Group B) Identification - Isolate

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Aliases

GBS, S. agalactiae, Streptococcus agalactiae

Test Name
Streptococcus (Group B) Identification - Isolate
Test Code

GBSID

Methodology

MALDI-TOF (matrix-assisted laser desorption ionization-time of flight) mass spectrometry

Overview/Supplementary

This test confirms the identification of group B Streptococcus. This test is for surveillance of invasive disease caused by group B streptococci.

Turn Around Time

Not Applicable

Specimen Requirements

Isolates: Actively growing culture from an invasive site on media that support the growth of the bacterial isolate submitted.

Special Considerations

For surveillance use only. Results not reported back to submitter. 

This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html).

Transport/Stability

Store and ship at ambient (8°C to 25°C) or refrigeration (2°C to 8°C) temperature.

Interface/Codes

LOINC: 586-8

Test Resource ID
939