Aliases
Carbapenem-resistant Pseudomonas, CP-CRPA, CRPA, KPC, NDM, VIM, IMP, and OXA-48
Submitted by
StewaD1
on
Test Name
Carbapenemase Detection in Pseudomonas - Isolate
Test Code
CRP
Methodology
16S Sanger sequencing, Biochemicals, Carba NP, Conventional PCR, MALDI-TOF (matrix-assisted laser desorption ionization-time of flight) mass spectrometry, Modified carbapenem inactivation method (mCIM), Real-time PCR
Overview/Supplementary
This test detects the following carbapenemase genes in Pseudomonas spp.: KPC, NDM, VIM, IMP, and OXA-48.
Turn Around Time
2-7 days
Specimen Requirements
Isolates: Actively growing culture on media that support the growth of the bacterial isolate submitted.
Isolates should be subcultured as few times as possible prior to submitting for testing to limit the potential for resistance plasmid loss during passage.
If a culture isolate(s) is submitted for testing, the original culture/isolate/inoculum should be maintained by the submitter until MDH results are reported.
Special Considerations
Submit copy of antimicrobial susceptibility testing results from clinical lab along with the isolate.If submitting isolate for AR Lab Network, please fill out ARLAB Central Region Clinical Testing and Submission form https://www.health.state.mn.us/diseases/idlab/arln.html.
This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html).
Transport/Stability
Store and ship at ambient (8°C to 25°C) or refrigeration (2°C to 8°C) temperature.
Interface/Codes
LOINC: 86930-5
Test Resource ID
881