Aliases
Shigellosis, EIEC/Shigella
Submitted by
StewaD1
on
Test Name
Shigella Culture - Stool
Test Code
RDRSPEC
Methodology
Biochemicals, Culture, Serology
Overview/Supplementary
This test isolates Shigella species from clinical specimens.
Culture-independent diagnostic tests (CIDTs), like BioFire FilmArray, target the ipaH gene to detect both Shigella and Enteroinvasive Escherichia coli (EIEC) but are unable to differentiate between the organisms.
MDH-PHL is utilizing PCR and whole genome sequencing (WGS) to conduct surveillance of Enteroinvasive Escherichia coli (EIEC) to gain a deeper understanding of the epidemiology of this pathogen.
Turn Around Time
3-7 days
Specimen Requirements
Specimens: Stool or rectal swabs preserved in Para-Pak C&S, modified Cary-Blair or equivalent transport media.
Minimum volumes:
Rectal swab: shows visible trace of fecal material and is immersed in transport media.
Stool specimen: transport media vial filled with approximately 5 ml of stool.
Special Considerations
This organism is reportable and submission of clinical materials is required by the MN Reportable Disease Rule 4605.7000 - 4605.7900 (https://www.health.state.mn.us/diseases/reportable/rule/index.html).
Transport/Stability
Store at ambient (8°C to 25°C) temperature. Ship at ambient (8°C to 25°C) or refrigeration (2°C to 8°C) temperature.
Interface/Codes
LOINC: 82300-5
Test Resource ID
937