Rhinovirus and Enterovirus Multiplex PCR | Minnesota Department of Health

Aliases

Enterovirus typing, EV68

Test Name

Rhinovirus and Enterovirus Multiplex PCR

Test Code

RVEVPCR

Methodology

Real-time multiplex PCR

Overview/Supplementary

This test detects and distinguishes rhinovirus from enterovirus RNA. Samples positive for enterovirus RNA may be further characterized with Enterovirus Genotype by VP1 NGS Sequencing.

Turn Around Time

5-7 days

Specimen Requirements

Specimens in DMEM, EMEM, Sterile PBS, Sterile Saline, UTM, or VTM: Bronchial wash, Bronchoalveolar lavage/BAL, Dual NP/OP swab, Nasal aspirate, Nasal swab, Nasal wash, Nasopharyngeal swab, Oropharyngeal swab, Sputum, Tracheal aspirate, or Vesicular fluid/skin lesion swabs

Specimens in sterile container: Cerebrospinal fluid/CSF, Dry swabs of vesicular fluid/skin lesions, Skin lesions or scabs, or Stool (raw or in Cary-Blair)

Isolates: Viral culture isolates

Preferred volume: 3 mL
Minimum volume: 200 µL

Special Considerations

None

Transport/Stability

Store and ship respiratory specimens, vesicle swabs/scabs in VTM/UTM, and stool at refrigeration (2°C to 8°C) temperature within 7 days of collection.

Store and ship cerebrospinal fluid/CSF at refrigeration (2°C to 8°C) temperature within 10 days of collection. Alternatively, ship frozen (≤0°C).

Store and ship dry samples at ambient (8°C to 25°C) temperature.

Interface/Codes

LOINC: 40991-2